Composite

Part:BBa_M50033:Design

Designed by: Cale Lester, Raquel Freeman, Tristan Yeung   Group: Stanford BIOE44 - S11   (2016-11-01)


U1_mTurquoise2, IPTG-induced, CAM resistant Thermosensor Construct


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 61
    Illegal PstI site found at 67
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 61
    Illegal PstI site found at 67
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 49
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 61
    Illegal PstI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 61
    Illegal PstI site found at 67
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No BsaI restriction sites. Under 2kb for synthesis using DNA 2.0.


Source

Neupert, J., Karcher, D., and Bock, R. 2008. Design of simple synthetic RNA thermometers for temperature-controlled gene expression in Escherichia coli. Nucleic Acids Research. 36(19): e124.

Bajar, B., et al. 2016. Fluorescent indicators for simultaneous reporting of all four cell cycle phases. Nature Methods.

References